Animal Disease Model Construction Platform--Technology Platform

Neurological Disease Models

Category	Modeling Method	Animal
Parkinson’s Disease Model	Peripheral or intracerebral injection of MPTP	Rat/Mouse
	Striatal-substantia nigra injection of 6-OHDA	Rat/Mouse
	Substantia nigra injection of rAAV9X virus expressing a-Synuclein	Rat/Mouse
Alzheimer’s Disease Model	Bilateral resection of the fornix-hippocampus using stereotactic neurosurgery	Rat
	Bilateral implantation of sterile 7mm electrode into hippocampus through the dura mater and 1mA direct current is applied for stimulation (also known as bilateral electrolytic basal nucleus model)	Rat
	Stereotactic injection of ibotenic acid	Rat
	Intraperitoneal injection of scopolamine	Rat
	Injection of Aβ solution into the hippocampal region	Rat
	Stereotactic injection of okadaic acid	Rat
	Transgenic animals (PS-1, PS-2, apoE4, etc.)	Rat/Mouse
	Stereotactic injection of immunotoxin for the elimination of basal forebrain cholinergic cells	Rat
Aging and Dementia Model	Subacute aging induced by D-galactose (D-gal)	Rat
	24-month-old (natural aging)	Rat
	Intragastric administration of aluminum hydroxide hexahydrate (dementia)	Rat
Cerebral Ischemia/Hemorrhage/Stroke Model	5 minutes’ bilateral carotid artery ligation followed by reperfusion/ligation of bilateral internal carotid and vertebral arteries followed by reperfusion (global cerebral ischemia)	Gerbil/Rat
	MCAO suture method (focal cerebral ischemia)	Rat
	Ferric chloride-thrombus in middle cerebral artery	Rat
	Intracarotid injection of a mixture of thrombogenic agents (adenosine diphosphate, thrombin, and adrenaline) causes platelet thrombus in capillaries and small arteries	Rat
	Stereotactic injection of autologous blood into specific brain regions	Rat/Mouse
	Stereotactic injection of non-heparinized autologous blood (multiple injections)	Rat/Mouse
	Heparin and collagenase mixed injection causes local hemorrhage	Rat/Mouse
	Injection of autologous blood mixed with cerebrospinal fluid into cisterna magna (autologous blood, fresh arterial blood, and oxygenated hemoglobin) [subarachnoid hemorrhage]	Rat
	Electric coagulation knife injury or stereotactic injection of paraffin oil (traumatic hemorrhage)	Rat/Mouse
Demyelination Model	Experimental autoimmune encephalomyelitis (EAE) induced by immunogens such as MOG (35-55), MBP (68-86), PLP (139-151), guinea pig spinal cord homogenate, etc.	Rat/Mouse
Depression Model	Forced swim test	Rat/Mouse
	Tail suspension test	Mouse
	DL-5-Hydroxytryptophan (5-HTP) induction	Mouse
	Reserpine induction	Mouse
	Yohimbine toxicity potentiation	Mouse
	High-dose apomorphine antagonism	Mouse
	Chronic moderate stress model (foot shock, cold water swimming, heat stress, tail clip, water and food deprivation, reversed light-dark cycle)	Rat/Mouse
	Novelty-suppressed feeding test	Rat/Mouse
Epilepsy Model	Injection of kainic acid (KA) for epilepsy model (systemic and intracerebral administration)	Rat/Mouse
	Injection of lithium-pilocarpine (lithium-pilocarpine model)	Rat/Mouse
	Stereotactic injection of kainic acid into the amygdala	Rat/Mouse
	Subcutaneous injection of pentylenetetrazol (PTZ) for acute epilepsy model	Rat/Mouse
Anxiety Model	Chlorpromazine induction (rat anxiety model)	Rat
Anxiety Model	Social stress induction (rat anxiety model)	Rat
Learning Model	Acquisition memory impairment	Mouse
	Impaired memory consolidation	Mouse
	Memory retrieval impairment	Mouse
	Light-dark box test	Mouse
Schizophrenia Model	MIA-801 (Dizocilpine maleate) injection	Rat
	Neonatal brain injury (neurodevelopment model)	Rat
	Catechol-O-methyltransferase gene (COMT) (transgenic schizophrenia model)	Rat
	Neuregulin 1 gene (transgenic schizophrenia model)	Rat
	Disrupted in schizophrenia 1 (DISC1) gene knockout	Rat
Pain Disease Model	Weight drop or contusion	Rat/Mouse
	Photochemical-induced spinal cord injury	Rat/Mouse
	Partial sciatic nerve ligation	Rat/Mouse
	Herpes zoster neural model	Rat/Mouse
	Diabetic neuropathic pain	Mouse
	Neurofibroma model	Rat/Mouse

The assessment of neurodegenerative disease modeling typically includes:

1) Cognitive function test

2) Motor function test

3) Immunohistochemistry analysis (GFAP, CHAT, TH, c-fos, etc.) and others.

Cognitive Function Test

Motor Function Test

Morris Water Maze

Passive Avoidance Test

Y-Maze

Elevated Plus Maze

Platform Jumping Test

Shuttle Box

Novel Object Recognition Test

Social Defeat Stress Test

Attention Set-Shifting Task

Conflict Drinking Test

Sucrose Preference Test

Open Field Test

Rotarod Test

Pole Climbing Test

Traction Test

Stepping Test

Wall Following Test

Adhesive Removal Test

Von Frey Test

Tail Suspension Test

Forelimb Placement Test

Additionally, our company also offers small animal in vivo imaging using MRI/PET-CT, tissue fluorescence imaging (including Celigo cell and tissue imaging and analysis, whole-brain slide scanning, tissue immunofluorescence imaging), patch clamps for cells and brain slices, multi-channel in vivo electrophysiological recordings in awake animals, respiratory monitoring, behavioral testing related to learning and memory, motor function, anxiety, depression, and sensory perception (go/no-go).

Endocrine Disease Models

Modeling Method	Animal
DIO Model	C57BL/6
DIO+STZ-induced T2MD	SD Rat
Spontaneous Diabetes	db/db Mice (BKS background) / ZDF Rat
B6-OB Spontaneous Obesity/Diabetes	ob/ob Mice
LEPR Gene Knockout	Mice

The assessment of diabetes/obesity modeling typically includes:

1) Glucose tolerance test (GTT) and insulin tolerance test (ITT): OGTT, IPGTT, IPITT, IVGTT, etc.

2) Immunohistochemical analysis (insulin/Glucagon/Ki67/Pdx-1) and TUNEL staining.

Respiratory System Disease Models

Category	Modeling Method	Animal
Idiopathic Pulmonary Fibrosis	Cav1 gene knockout	Mouse

The assessment of respiratory system disease modeling typically includes:

1) Lung tissue histopathological examination (H&E staining, Masson staining).

2) Immunohistochemistry (IHC) testing, which may involve specific markers such as alpha-smooth muscle actin (a-SMA) for fibrosis assessment.

Liver Disease Models

Category	Modeling Method	Animal
Wilson’s Disease (WD)	Atp7b gene knockout	Mouse
Non-Alcoholic Steatohepatitis (NASH)	Hif1 gene knockout	Mouse

The assessment of liver disease modeling typically includes:

1) Liver tissue histopathological examination (H&E staining, Oil Red O staining, Masson’s trichrome staining).

2) Serum biochemical marker detection (AST, ALT, LDL-C, etc.).

Rheumatoid Arthritis Models

Category	Modeling Method	Animal
Inflammatory Bowel Disease	NOD2/TLR2 gene knockout	Mouse
Rheumatoid Arthritis	IL1-ra gene knockout	Mouse
Experimental Autoimmune Encephalomyelitis	CD274 gene knockout	Mouse
Psoriasis	Transgenic animal (STAT3C gene)	Mouse

The assessment of inflammatory/immune-mediated disease modeling includes:

1) Histopathological examination (H&E staining, toluidine blue staining, and Safranin O staining).

2) Immunohistochemistry testing (iNOS, IL-17, TNF-α, IL-1β, IL-6, and others).

3) Monitoring of body weight changes over time.

4) Disease scoring, organ length measurements, and body weight measurements.

5) Blood biochemical analysis, including leukocyte classification, interleukin analysis, and histopathological scoring.

Cardiovascular Disease Models

Category	Modeling Method	Animal
Atherosclerosis	LDLR gene knockout	Mouse
Hemophilia A	F8 gene knockout	Mouse

The assessment after atherosclerosis modeling typically include:

1) Aortic arch Oil Red O staining.

2) Biochemical analysis (TC, TG, HDL-C, and LDL-C).

Other Metabolic Disorders Models

Category	Modeling Method	Animal
Bardet-Biedl Syndrome	BBS5 gene knockout	Mouse
Mucopolysaccharidosis Ⅳ	Galns point mutation	Mouse
Mucopolysaccharidosis Ⅱ	IDS point mutation	Mouse
Lipid Storage Disorder	Gnptab point mutation	Mouse
Mucopolysaccharidosis Ⅰ	Point mutation	Mouse

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